FISH POPULATION DYNAMICS OF ANNUALLY-FLOODED SEASONALLY-ISOLATED BACKWATER LAKE OF THE ILLINOIS RIVER Matt Stroub and Greg G. Sass Illinois River Biological Station, Illinois Natural History Survey, 704 N. Schrader Avenue, Havana, Illinois 62644 Connectivity and isolation from rivers may be major drivers of fish community composition in backwater lakes. Matanzas Lake is a seasonally-isolated backwater lake of the La Grange reach of the Illinois River. Matanzas Lake was flooded and connected to the Illinois River from late February to mid- May, 2007. From mid-May to present, Matanzas Lake has been isolated from the Illinois River. Weekly, we used a multiple-gear approach to sample the fish community of Matanzas Lake both pre- and post-isolation from the Illinois River. We used single and tandem fyke nets, trammel nets, experimental gill nets, and day electrofishing at fixed sites to assess the fish community of Matanzas Lake over time. All fish captured were identified to species, measured, and weighed. Basic water quality information (e.g. water temperature, dissolved oxygen) was also collected at all fixed sites on each sampling date. Our objectives were threefold: 1) to test for differences in fish species community composition and catch rates from Matanzas Lake pre- and post-isolation from the Illinois River; 2) to test for differences in fish species diversity among the La Grange Reach of the Illinois River and Matanzas Lake; and 3) to identify unique fish species to Matanzas Lake. Electrofishing catch rates of the ten most common fishes, except emerald shiner Notropis atherinoides Rafinesque, were greater during isolation from the Illinois River. Eight of ten fish species showed statistically greater electrofishing catch rates. Fish species diversity of Matanzas Lake in 2007 was greater than the 17 year LTRMP fish species diversity average of the La Grange reach of the Illinois River. There was also six species of fish that we considered unique to Matanzas Lake, orange spotted sunfish Lepomis humilis, grass pickerel Esox americanus vermiculatus, mooneye Hiodon tergisus, Yellow Bass Morone mississippiensis, red shiner Notropis lutrensis and green sunfish x bluegill. We are uncertain if conductivity to the main stem river has affect on the fish diversity and fish community of these important backwater lakes.