HISTORICAL PERSPECTIVE PF TROPHIS STRUCTURE OF THE LOWER OHIO RIVER USING STABLE ISOTOPES Lindsey Rice1, Michael D. Delong1, James H. Thorp2, and Jeffrey R. Anderson3 1Large River Studies Center, Biology Dept., Winona State University, Winona, MN 55987.2Kansas Biological Survey, Dept. Ecology and Evolutionary Biology, University of Kansas, Lawrence, KS 66407. 3Large River Studies Center, College of Science and Engineering, Winona State University, Winona, MN 55987 Most recently have we seen and studied the profound effects humans have on ecosystems. The lower Ohio River ecosystem has been vastly influenced by the actions of humans (locks and dams, agricultural runoff, and urban/industrial discharge) for decades. This study is an in-depth examination to determine how and to what extent human disturbances have affected riverine trophic positions and length of food chain of the lower Ohio River. Museums contain specimens (fish, freshwater snails, and freshwater mussels) from present to historic time periods and we can use tissue from these specimens to identify changes in stable isotope ratios of carbon and nitrogen in order to help determine changes/shifts in trophic levels. The nitrogen ratio increased around 1970 for piscivores but stayed rather consistent for the other trophic positions. Carbon stable isotope ratios slowly decreased from 1920 to 2000. Trends in both nitrogen and carbon stable isotope ratios may be correlated with human disturbances and its effect on the river’s natural ecosystem. The data collected will not only address the extent of the changes in the ecosystem/food webs, but may also give consideration as to if the river’s ecosystem is past the point of rehabilitation. Keywords: food web, stable isotope, food chain, disturbance, temporal Lindsey L. Rice Large River Studies Center, Biology Dept., Winona State University, Winona, MN 55987 (507)-457-2458 LLRice0752@winona.edu Poster presentation format only Student