EFFECTS OF FLOOD TIMING AND SPATIAL DISTRIBUTION ON NITRATE EXPORT FROM THE UPPER MISSISSIPPPI BASIN David M. Soballe1, Dennis M. Wasley2, and Richard H. Coupe3 1Environmental Laboratory, Army Engineer Research and Development Ctr, 3909 Halls Ferry Rd, Vicksburg, MS 39180, 2Minnesota Pollution Control Agency, 520 Lafayette Road North, St. Paul, MN 55155-4194. 3U. S. Geological Survey, 308 South Airport Road, Pearl, MS 39208-6649 We examined the transport of nitrate + nitrite nitrogen (nitrate) in the Mississippi River during the period 1992-2001 with emphasis on two major floods (1993 and 2001) in the Upper Mississippi River basin (above the Missouri River confluence with the Mississippi River). We found significant influences of flood frequency (antecedent conditions), seasonal timing, and geographic distribution of flooding on nitrate transport. The results show that major floods dominate the movement of nitrate in this system and that the spatial and temporal distribution of flooding has substantial influence on nitrate transport. Of particular importance to the concentration of nitrate in flood waters is the level of discharge from the basin during the preceeding year. The character of flood waters delivered from the Upper Mississippi Basin to the Gulf of Mexico can also be strongly influenced by dilutional flows from other basins in the Mississippi drainage (e.g., the Ohio River) so that the seasonal timing of Upper Mississippi floods relative to flooding in other subbasins can have important consequences. Our results show that the size of the summer hypoxic zone in the Gulf of Mexico, which varies in relation to discharge from the Upper Mississippi River, appears particularly sensitive to May discharge from the Upper Mississippi. keywords: nitrogen, Gulf hypoxia, antecedent conditions, loading