EVALUATION OF A BARRIER TO PREVENT TRANSFERS OF HARMFUL AQUATIC SPECIES BETWEEN THE MISSISSIPPI AND GREAT LAKES BASINS. Traci L. Barkley 1, Richard E. Sparks 1, John M. Dettmers 2 1University of Illinois, Natural Resources and Environmental Science, Urbana, IL 61801, 2Illinois Natural History Survey, Lake Michigan Biological Station, Zion, IL, 60099. The Chicago Sanitary and Ship Canal, located in northeastern Illinois, links two of the largest drainage basins in North America: the Mississippi and the Great Lakes-St. Lawrence and has become a gateway for the transfer of aquatic nuisance species between the two basins. Nuisance organisms that have moved through the canal from Lake Michigan to the Mississippi drainage in the past decade include the zebra mussel (Dreissena polymorpha) and the round goby (Neogobius melanostomus). Other nonindigenous fish are poised to enter the Mississippi River Basin by way of the canal, such as the European river ruffe (Gymnocephalus cernuus). The ruffe is a small, spiny fish that is neither a desirable forage item for other fishes, nor a commercial or sport species and is likely to compete with highly-valued species. The ruffe entered northern Lake Michigan in September 2002 and will eventually work its way south along the shoreline to Chicago and the canal system. Kolar and Lodge (2002), using a modeling approach to assess risk to the Great Lakes from potential unintentional ballast water introductions from the Ponto-Caspian region, predict five more fishes will become nuisance species. Currently moving in the opposite direction, two Asian fishes, the bighead carp (Hypophthalmichthys nobilis) and the silver carp (Hypophthalmichthys molitrix) are rapidly approaching Lake Michigan via the canal. An electric dispersal barrier was constructed by the U.S. Army Corps of Engineers in the canal and activated 9 April 2002. It provides an opportunity for rapid assessment and development of barrier technology. Because the barrier is experimental, its performance needs to be assessed promptly to optimize field strength, configuration, and the electric pulse rate before the impending arrival of the Asian carps and the European ruffe. A combined radio and acoustic telemetry system tracks movements of fish, implanted with transmitters, in the vicinity of the barrier. The transmitters emit coded sonic and radio signals so individual fish can be identified and movements from one side of the barrier to the other can be detected. Barge traffic and high flow events, which may affect the electric field and the movement of fish, are monitored with a web- accessible video camera, hydrophones, and several U.S. Geological Survey acoustic velocity meters mounted just downstream of the barrier. If any tagged fish move through the barrier, barrier operators will be notified and the microprocessor-controlled electric field can be modified. Each modification of the electric field is tested by the introduction and tracking of tagged fish. Once proven in the field, this electric barrier technology could be used to retard or prevent invasions of unwanted fishes, not only in the Chicago Sanitary and Ship Canal, but wherever there are interbasin connections. Keywords: aquatic nuisance species, Chicago Sanitary and Ship Canal, dispersal barrier, monitoring, telemetry.