MULTIPLE QUANTITATIVE UNIONIDAE SURVEYS OF THE SAME TRANSECT WITH SPECIMEN REMOVAL BETWEEN SURVEYS; CHIPPEWA RIVER, WISCONSIN Chris Wallace and Terry Balding. University of Wisconsin-Eau Claire, Biology Department, Eau Claire, WI 54702 During the summer of 2001 we conducted 4 consecutive unionid surveys along the same transect on the Chippewa River, Wisconsin. We relocated all unionids after each survey. Our purposes in this study were to determine 1) if there was any vertical movement by unionids during normal water levels, 2) if smaller individuals would be recovered in later swim-over surveys, and 3) if there were differences in the sizes or species collected by swim-over versus digging surveys. From the 4 consecutive surveys of the same transect there were a total of 18 species found and 542 live unionids were counted (density of approximately 2.8 unionids/m2). Among the unionids collected were two Wisconsin Threatened and Endangered species, Plethobasis cyphyus and Tritigonia verrucosa. The dominant species were Potamilus alatus and Fusconaia flava, comprising nearly 57.4% of all the unionids. The data from this study provide some indication that within a 46-day period unionids may move vertically within the substrate. The data also show there is a significant difference in the mean standardized lengths for the 3 consecutive surveys (R2 = 0.9984, P ( 0.05). While there are only 3 data points and the range in size is slight, the data indicate swim-over surveys have a bias for larger unionids. Overall we found smaller unionids and a greater number during the digging survey than the swim-over survey. Additionally, some species and some sizes were under-represented during the swim-over survey compared to the digging survey. These findings indicate surveys intending to obtain community and population structure should use digging.